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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong, F. Ann Ran, David Cox, Shuailiang Lin - Science - 2013Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to i…被引用次数：15,402Genome engineering using the CRISPR-Cas9 system
F. Ann Ran, Patrick D. Hsu, Jason Wright, Vineeta Agarwala - Nature Protocols - 2013该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：11,426The new frontier of genome engineering with CRISPR-Cas9
Jennifer A. Doudna, Emmanuelle Charpentier - Science - 2014The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery through the elucidation of the CRISPR-Cas9 enzyme mechanism, which has set the stage for remarkable developments using this technology to modify, regulate, or mark…被引用次数：6,692CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Rodolphe Barrangou, Christophe Fremaux, Hélène Deveau, Melissa Richards - Science - 2007Clustered regularly interspaced short palindromic repeats (CRISPR) are a distinctive feature of the genomes of most Bacteria and Archaea and are thought to be involved in resistance to bacteriophages. We found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences. Removal or addition of particular spacers modified the phage-resistance phenotype of the cell. Thus, CRISPR, to…被引用次数：6,161Development and Applications of CRISPR-Cas9 for Genome Engineering
Patrick D. Hsu, Eric S. Lander, Feng Zhang - Cell - 2014该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：5,727Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Ophir Shalem, Neville E. Sanjana, Ella Hartenian, Xi Shi - Science - 2013The simplicity of programming the CRISPR (clustered regularly interspaced short palindromic repeats)-associated nuclease Cas9 to modify specific genomic loci suggests a new way to interrogate gene function on a genome-wide scale. We show that lentiviral delivery of a genome-scale CRISPR-Cas9 knockout (GeCKO) library targeting 18,080 genes with 64,751 unique guide sequences enables both negative and positive selection…被引用次数：5,371Nucleic acid detection with CRISPR-Cas13a/C2c2
Jonathan S. Gootenberg, Omar O. Abudayyeh, Jeong Wook Lee, Patrick Essletzbichler - Science - 2017Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral effect of Cas13a with isot…被引用次数：3,591Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Lei S. Qi, Matthew H. Larson, Luke A. Gilbert, Jennifer A. Doudna - Cell - 2013该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：5,031Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
Bernd Zetsche, Jonathan S. Gootenberg, Omar O. Abudayyeh, Ian M. Slaymaker - Cell - 2015该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：4,705Improved vectors and genome-wide libraries for CRISPR screening
Neville E. Sanjana, Ophir Shalem, Feng Zhang - Nature Methods - 2014该记录暂无摘要，您可以通过来源链接查看详细信息。被引用次数：5,338